- Giant Spider
- Dr. Hare
- Director D
- Copy Cat
- Speeding Spike
- Sir Rebral
- Ratman
- Crusher
- Betty Jetty
- Vince Graves
- Mordred
- Black Widow
- Zeus
- Medusa
- Captain Crawfish
- Boss Plant
- Gretchen Grimlock
- El Mustachio Grande
- The Shogun
- Mr. Silva
- Mademoiselle Moreau
- Daphne Dreadnaught
- Magistrate Henry Flatbottom
- Burt Diamond
- Samuel Brains
- Preston Wilhelm
- Black Bandit
- CC13 Influenza
- Myron Van Buren
- Octavian
- Erik the Red
- Booted Bandit
- Red Baroness
- E.Vile
- The Bully
- Dr. Cumulo Nimbus
- Arthur Eraser
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List of Villains
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